Protocol for the assessment of mTOR activity in mouse primary hepatocytes

Protocol for the assessment of mTOR activity in mouse primary hepatocytes

Blog Article

Summary: We present a protocol for THE VOLATILITY SPILLOVER EFFECT OF COVID-19 ON INVESTOR SENTIMENT IN JOHANNESBURG STOCK EXCHANGE measuring the activity of the mechanistic target of rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes.It can be used as a tool for genetic, pharmacological, metabolomic, and signal transduction procedures.We discuss critical aspects for improving yield, viability, and modulation of the mTOR pathway.This protocol can be adapted to other signaling cascades and is compatible with multiple readouts.

For complete details Strong Time-Consistent Solution for Cooperative Differential Games with Network Structure on the use and execution of this protocol, please refer to Ortega-Molina et al.(2021).